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KMID : 0368420070500050557
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Journal of Plant Biology
2007 Volume.50 No. 5 p.557 ~ p.561
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Isolation of new microsatellite-containing sequences inAcanthopanax senticosus
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Kim Joon-Ki
Chung Ki-Wha
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Abstract
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Microsatellite markers, also called simple sequence repeats (SSRs), are comprised of a 2-to 6-nucIeotide repeat motif. They are useful as molecular markers for genetic authentication, crop breeding programs, and linkage analysis for map-based cloning. From a microsatellite-enriched genomic library ofAcanthopanax senticosus, we identified 239 new microsatellite-containing sequences. The di-nucleotide repeat units were the most abundant (55.2%), followed by tri-nucleotide repeat units (24.6%). In detailed repeat structures, the (AG)n motif was most frequent (30.5%), followed by the (AC)n motif (21.7%). Heptaand octa-nucleotide repeat motifs were found in each single locus, and a total of 33 (13.8%) complex repeat structures were recorded. This is the first report of mass isolation of microsatellites via screening of anA. senticosus library, and may well provide information useful as a genetic resource for the further study ofA. senticosus.
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KEYWORD
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Acanthopanax senticosus, genetic marker, microsatellites, simple sequence repeats (SSRs)
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